Aldo Barrera Vásquez

4 Questions 5 Answers 0 Followers

Questions related from Aldo Barrera Vásquez

Does Promega's Passive Lysis 5X Buffer contains anti-protease and anti-phosphatase components?

Hi everyone, Do you know if the Passive Lysis 5X Buffer that Promega produce has these components? I always use it to lysate transfected cells and measure lucierase activity and now I'm wondering...

09 September 2018 9,505 1 View

Early response genes detection to osmotic, oxidative and DNA damage induction in cells?

Dear colleagues, I am working on the detection of multiple stress conditions induced in cell culture (HEK293T and HeLa cells) as osmotic (sorbitol/NaCl), oxidative (arsenite) and DNA damage...

05 May 2018 5,121 3 View

An efficient probe for the detection of HCV RNA?

Hi everyone, I want to design a protocol to detect viral RNA of Hepatitis C virus though real time PCR with probes. I used to use a regular qPCR with primers against the 5'UTR region (most...

01 January 2017 752 0 View

QRTPCR amplification of target with low GC content?

Hey everyone. I have a question. I'm doing the in vitro silencing of a small RNA of 79 bp (a snoRNA specifically), and I need to design a protocol for the detection of the known-down of this RNA....

12 December 2016 6,294 3 View