Alba Bellot Saez

6 Questions 7 Answers 0 Followers

Questions related from Alba Bellot Saez

What is the best way to measure the M-current from Layer V pyramidal neurons in cortical slices?

Hi, I have been trying to measure the m-current in several pyramidal cells in the cortex in voltage-clamp mode using a protocol from -30mV to -70mV, before and after TTX and XE991. However most...

03 April 2017 9,860 2 View

What is the best way to silanize selective barrels to build K+ sensitive electrodes for extracellular recordings?

We are interested in studying K+ dynamics in cortical slices and to do so we will perform extracellular recordings with K+ sensitive electrodes. However, we have got some problems in buildind...

19 May 2016 8,744 1 View

Is GAP-134 a specific activator of gap junctions in astrocytes?

I would like to use the GAP-134 gap junction activator to study K+ dynamics in cortical slices. However, I am not sure if it is purely specific for astrocytes of it also activates gap junctions in...

27 April 2016 3,258 3 View

Are astrocytes the most abundant subtype of glia in the CNS or the numbers are still controversial?

I have some doubts about the numbers of glial cells in the CNS since most articles have claimed during the past decades that astrocytes are the most abundant cell type in the brain, but there are...

28 March 2016 8,966 5 View

Is 30x a good microscope magnification to record VSD signals from rodent cortical slices or it would be better to use a smaller one?

I'm trying to set the microscope and cameras up in order to record VSD signals from rodent cortical slices stained with voltage-sensitive dyes (DI-4-ANEPPS; RH1691). I would appreciate any...

17 March 2016 9,115 1 View

Is RH-1691 a good sensitive voltage dye for in situ recordings in rodent cortical brain slices?

Hi all, I’m planning on performing voltage-sensitive dye imaging experiments in cortical brain slices to study neuronal-astrocytic interactions. Since blue dyes have longer wavelengths...

02 March 2016 984 13 View