Alba Bellot Saez

5 Questions 7 Answers 0 Followers

Questions related from Alba Bellot Saez

What is the best way to measure the M-current from Layer V pyramidal neurons in cortical slices?

Hi, I have been trying to measure the m-current in several pyramidal cells in the cortex in voltage-clamp mode using a protocol from -30mV to -70mV, before and after TTX and XE991. However most...

03 April 2017 9,835 2 View

What is the best way to silanize selective barrels to build K+ sensitive electrodes for extracellular recordings?

We are interested in studying K+ dynamics in cortical slices and to do so we will perform extracellular recordings with K+ sensitive electrodes. However, we have got some problems in buildind...

19 May 2016 8,701 1 View

Is GAP-134 a specific activator of gap junctions in astrocytes?

I would like to use the GAP-134 gap junction activator to study K+ dynamics in cortical slices. However, I am not sure if it is purely specific for astrocytes of it also activates gap junctions in...

27 April 2016 3,237 3 View

Is 30x a good microscope magnification to record VSD signals from rodent cortical slices or it would be better to use a smaller one?

I'm trying to set the microscope and cameras up in order to record VSD signals from rodent cortical slices stained with voltage-sensitive dyes (DI-4-ANEPPS; RH1691). I would appreciate any...

17 March 2016 9,089 1 View

Is RH-1691 a good sensitive voltage dye for in situ recordings in rodent cortical brain slices?

Hi all, I’m planning on performing voltage-sensitive dye imaging experiments in cortical brain slices to study neuronal-astrocytic interactions. Since blue dyes have longer wavelengths...

02 March 2016 958 13 View