Alaleh Rezalotfi

6 Questions 16 Answers 0 Followers

Questions related from Alaleh Rezalotfi

How much protein (microgram) can be extracted from 15 ml of a cancer cells line conditioned medium?

I'm going to extract total protein from 15 ml of a cancer cell line 24-hour conditioned medium. I need to know how much protein I can extract from this amount of CM, and is this enough for mass...

12 December 2018 9,007 8 View

How flowcytometry data should be reported?

I am wondering if we have to consider percentage of Treg (CD4+CD25+FoxP3+) from total lymphocyte gate or the percentage from CD4+CD25+ gate?

09 September 2018 5,584 3 View

Are there any journals that only publish ideas and hypotheses?

Is it worth to publish ideas and hypotheses? And are there any journals that only publish ideas without data?

03 March 2018 2,498 12 View

Is it necessary to treat cancer cell lines with mitomycin to extract conditioned media ?

I would like to treat PBMCs with cancer cell lines conditioned medium (CM) to observe its effect on PBMCs. So far, I have treated cell lines with 10 micro g/ml in serum free RPMI (FBS limits...

01 January 2018 1,807 5 View

Can both surface and intracellular staining be done in the intracellular staining process?

Is it possible to stain cells with both surface and intracellular antibodies in one step after fixation and permeabilization ? I think it makes the staining procedure shorter and may improve cell...

08 August 2017 9,441 11 View

Any alternative procedure for intracellular staining ?

I have tried two protocols for intracellular staining of PBMCs after culture: 1- Manual method: after staining with surface Abs, fix cells with 100 micro litter PFA 4% and incubate for 20 minutes...

01 January 1970 2,941 11 View