8 Questions 14 Answers 0 Followers
Questions related from Alaa Ibrahim
Hi all, I have a Knock out mouse' spinal cord (SC) fixed in tissue tek, and it was treated before with PFA, and sucrose (for using it in IHC experiment). However, I need to do a genotyping...
30 October 2017 2,639 10 View
I'm always wondering why some antibodies are only suggested to be applied to paraffin-embedded section, but NOT frozen section. In my eyes, if the antibody could reach the antigen on paraffin...
02 March 2017 5,698 7 View
I want to do a double immunofluorescence staining, and I am wondering how to choose the secondary antibody, if both of the primary antibodies are from the same host; rabbit. In other words, I...
21 February 2017 6,199 7 View
Hi all! Can a mouse whole vertebral column be stored in 4% PFA for longer than 24 hours, but shorter than one week? I plan to put the vertebral columns of my mice in PFA on a Friday and do sucrose...
08 September 2016 8,254 9 View
Dear all, Your help is needed here: We have a list of 700 proteins: is it possible to find out, which ones are relevant for pain and inflammation without having to check them manually? Something...
13 April 2016 2,832 3 View
Dear all, Your kind advise is needed here: I am working with mice- in which we initiate a trauma for inflammation activation. I am planning to block one of the anti-inflammatory cytokines'...
07 April 2016 1,915 4 View
Hi, To extract RNA properly out of a whole Skin sample (mouse), how can I homogenize the sample? would the ceramic beads be enough for that? and later, which is better, TriFast or Trizol? Thanks...
25 November 2015 1,057 3 View
I want to perform IHC assay on tissues extracted from mice. My question: Is it better to perfuse the mouse first before extracting the tissues? or to extract the tissues first then fix them...
04 November 2015 7,803 7 View
