Alaa Hassanien

6 Questions 2 Answers 0 Followers

Questions related from Alaa Hassanien

Can I use stored working solution of Qubit dsDNA BR Assay Kits?

I need to know if I can prepare and store the working solution at 4°c overnight. I mean without adding any sample to it, only the br reagent and br buffer.

06 February 2022 9,460 2 View

Why there is a difference in the reading of spectrophotometer and microplate reader?

I measured the OD of my samples using spectrophotometer (750 nm), and microplate reader, and there was a difference between them.. so when do I consider the difference a minor and neglectable.....

25 August 2021 1,486 2 View

How and when do I add fertilizer in fertilizer testing experiment?

I am testing the effectiveness of dried algae biomass as a bio-fertilizer compared to the chemical fertilizer NPK, The plant I'm using is cherry tomatoes, they are about 25 days old. What is the...

25 November 2018 3,512 8 View

How to amplify gene without DNA extraction?

I have transformed agro-bacterium, and I need to amplify the introduced gene (Aska) using PCR without extracting the DNA, I have the bacteria sample as both freezed at -80C in glycerol stock and...

10 September 2018 4,145 9 View

How does grinding affect plant DNA extraction?

I grinded callus for DNA extraction, but I couldn't make it into very fine powder, to how extent that would affect the extraction procedure

28 March 2018 4,204 3 View

Which antibiotic is better to treat infected plant?

I am working on agrobacterium mediated transformation on tomato and I usually use meropan (50 ppm) for treating the plant after infection but i found that the plant is not doing well after...

26 March 2018 5,263 10 View