Akihiro Fukushima

4 Questions 13 Answers 0 Followers

Questions related from Akihiro Fukushima

Why do my neurons get hyperpolarized soon after break-in?

Hello there, I'm trying current-clamp recording from rat hypothalamic slices. In the literature, the hypothalamic neurons should fire spontaneously, but almost all of my neurons go deep (-80~-90...

19 July 2019 9,449 12 View

How should I analyze and interpret the data of the elevated plus maze test?

Hi everyone Now I’m trying to find behavioral phenotypes of mice (hereafter mice X) with the elevated plus maze, but behavioral tests are new for me. I followed the protocols described in...

04 July 2017 9,847 5 View

Does anyone mind telling me how to get A-type potassium currents in mouse CA1?

Hi everyone Now I'm trying to test effects of drugs on A-type potassium currents with mouse CA1 slices. I have conducted experiments on synaptic transmission (EPSC, IPSC), but the channel...

03 February 2017 1,973 3 View

How do you reduce the spike noise in the patch-clamp rig?

Hi everyone. In my patch-clamp rig, spike noises occur everytime suction tubes are renewed.(Please see the attached)I usually flush with a large volume of water or ACSF, but it takes time. Does...

26 January 2017 4,276 4 View