Aimilia Lydia Kalafateli

5 Questions 17 Answers 0 Followers

Questions related from Aimilia Lydia Kalafateli

Why do I get a double band on my Western Blot?

Hi everyone, I'm getting a band (probably unspecific binding), under my housekeeping COXIV protein. I think it's probably secondary antibody cross reaction, but I'm not entirely sure. On the...

10 October 2018 4,076 3 View

How could I possibly investigate if a hormone crosses the BBB?

Dear all, I am currently working with ghrelin (a gut peptide) and I would like to check if the peptide crosses the blood brain barrier and if it is possible to find out in which areas it reaches....

03 March 2016 705 6 View

CDNA synthesis: how can I dilute low RNA concentration of 66 ng/ul for a 1ug reaction?

Hello everyone, I just completed RNA extraction from brain tissue (PFC) using the RNeasy Lipid Mini kit from QIAGEN. One particular sample gave a very low concentration of 66 ng/ul RNA (in a total...

12 December 2015 4,798 8 View

Is BDA conjugated with fluorescein reliable for neuronal tracing?

I am a bit new to neuronal tracing and I want to use BDA for some frontal cortex areas of the rat brain. I want to use BDA conjugated with fluorescein, since the microscope I have in the lab is...

03 March 2014 3,264 2 View

Which is the best antibody for detection of phospho-p70S6K used in Western Blots?

I am doing some Western Blots and I want to trace bands of the phosphorylated p70S6K in mice brain tissue. I have repeated all my experiments more than 4 times and I still get no or very low...

08 August 2013 492 8 View