Adam K A Wright

4 Questions 6 Answers 0 Followers

Questions related from Adam K A Wright

Anti-human DP1 antibody?

Can anyone recommend a good anti-human DP1 (type-1 prostanoid receptor) antibody for either flow cytometry use? I appreciate there might be antibodies out there not validated for flow cytometry so...

07 July 2019 9,461 5 View

Is Bambanker a good cryogenic solution for storing PBMCs in -80 and Liq N2?

We have recently utilised Bambanker (BB01) from Nippon Genetics (Geneflow in UK), which is a serum free DMSO containing cryogenic storage medium for cells. PBMCs (5x10e5/ml) were not happy in...

09 September 2018 7,976 3 View

Has anybody been able to operate FlowClean, downsample and t-SNE in FlowJo (latest version for PC)?

I have got Attune NxT flow cytometry data in the latest version of flowJo (PC) that I wish to use FlowClean, downsample and t-SNE on. All three are listed in my plug-ins and when selected none of...

07 July 2017 9,944 7 View

Do human CD14++ monocytes also express CD34?

I have been using a CD34-PEcy7 reagent from Becton Dickinson (clone 581, class III epitope) to stain PBMCs for flow cytometric analysis. CD34 haematopoietic stem cells are not the only cells to...

03 March 2015 6,352 9 View