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Questions related from Abhishek Dubey
Hello everybody I had been doing semi-dry transfer in past few days and have experienced a persistent problem. I set-up my semi-dry transfer as follows: 1. Run a 12% SDS-PAGE gel under normal...
09 January 2022 4,083 2 View
Dear all I wanted to validate some of the points that I have heard about RNA extraction using Trizol methods. For some of them, I could not find justification online. I wanted to know if they are...
16 November 2021 2,280 5 View
Hello everyone. I am a beginner in phage display of antibodies. We have an in-home prepared phage display antibody library. I performed biopanning against an antigen, a purified protein, with...
06 September 2021 8,138 3 View
Hello everyone. I was wondering if it is possible to reconstruct complete field of 1 well of a 96-well plate if we have 3 or 4 images covering the entire field ? Basically, even though I could...
13 August 2021 4,134 3 View
I am attempting to purify a 34kDa protein that makes lots of inclusion bodies in E. coli. While isolating inclusion bodies, I noticed some of the protein also comes out in lysis buffer ( 10mM...
11 August 2021 5,289 2 View
Hello everyone. I was trying to clone a region of ULBP1 and MICA gene from human PBMC cDNA preparation. I had setup first PCR and even though I got the amplification, both of them are at wrong...
23 July 2021 3,311 6 View
Dear all I am facing a problem with plasmid recombination, even in Stbl3. The construct that I am trying to create is basically NFAT3x RE upstream to a GFP so as to make a NFAT-reporter cell...
28 March 2021 3,209 2 View
Dear all I hope you are having a good time. I am trying to ligate a PCR product of ~1500bp into a 6.5kbp vector (6.023kbp upon digestion). Somehow, I am not able to get colonies at all ! The...
08 March 2021 3,643 7 View
For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...
24 February 2021 7,324 4 View
I used a Ni-NTA BLI sensor. One protein was immobilized on the sensor. A pool of protein ( out of which one protein binds the immobilized protein ) is then used in the association phase. After...
30 June 2020 162 0 View
Recently, I have been confused because of conventions used in voltage clamping as opposed to current clamping. Under the voltage clamping conditions, inward movement of positive ions is considered...
01 January 1970 2,070 5 View
