Abhijit Chakraborty

5 Questions 6 Answers 0 Followers

Questions related from Abhijit Chakraborty

Any good methods for double-stained markers quantifications from tissue sample using computer programs?

How can we best quantify double-stained markers when comparing diseased and control? For example, if we want to stain one marker of smooth muscle cells and compare the expression in diseased and...

22 December 2020 7,539 1 View

What is the best procedure to deliver peptide- protein complex in cells (HEK293)?

Can anyone suggest the best procedure to make the protein-peptide complex and its transfection invitro? If directly add the complex to cell media is it works fine? How long I need to incubate?...

13 August 2020 5,070 1 View

What changes should i do to get contamination free cytoplasmic and nuclear protein extracts from mice Lung and Brain tissue?

I am trying to separate cytoplasmic and nuclear extract from Mice Lung and Brain tissue. Lung tissue sometime give clear protein. But Brain always get contamination. Even some time Nuclear protein...

09 August 2020 8,710 1 View

How can I confirm that a sequence variation is a mutation or Polymorphism?

I found a new variant (a substitution) in exon 20 of BRCA1 gene. This is not reported in HGVS, BIC or any other mutation database. The variant was found after sequencing with both the forward and...

27 April 2016 2,608 11 View

How do I calculate the annealing temperature of my PCR reaction?

Tm value of one of my primer is 52.8 (50mM NaCl) and another one is 54.5 (50mM NaCl) as given by the primer making company. Primer Sequence is – i> 5' TGT GTA TCA TAG ATT GAT GCT TTT GA 3' and...

14 June 2013 7,394 60 View