Abdullah Mahmud-Al-Rafat

9 Questions 11 Answers 0 Followers

Questions related from Abdullah Mahmud-Al-Rafat

What is the molecular weight and size of Rotavirus (whole virus)?

Can anyone give me the molecular weight and size of Rotavirus strain? Not the weight, size of proteins please.

09 September 2016 6,041 0 View

Can we use '1 M ortho phosphoric acid' for cell culture media pH adjustment instead of 1M HCl?

Usually 1M HCl is added to adjust pH in cell culture media containing bi-carbonate. However there are some argue about stability of pH adjusted by HCl. Some suggest that, ortho phosphoric acid...

07 July 2016 3,517 2 View

Is there any articles showing that, soluble interleukin 6 receptor (sIL-6R) induce more IL-10 expression than membrane bound IL-6 receptor?

Il-6/sIL-6r compound plays as pro-inflammatory while Il-10 plays as anti-inflammatory. But there's a connection between these two cytokines. Some evidence says that Il-6 increase IL-10 production...

04 April 2016 9,790 4 View

Is there any evidence based peer review article on dengue 5th serotype?

Since 2013, I'm hearing about dengue 5th serotype. There was an article by Dennis Normile in 'Science Magazine'. In this year a new article was also...

09 September 2015 3,475 0 View

Is 'Plasmocin™ by Invivogen' efficient to remove mycoplasma from virus stock?

It will be helpful if someone share their experience with plasmocin to remove mycoplasma from viruses. According to this article...

08 August 2015 4,324 3 View

What is the standard protocol for vero cell karyotyping? Can anyone please provide me any guidelines?

I have to do a karyotyping test for vero cell but I didn't find any standard protocol. Is there any laboratory kit available for vero cell karyotyping? Please help me to find these informations.

05 May 2015 1,728 1 View

Can anyone suggest the treatment steps if mycoplasma positive is found in biological samples e.g: FBS, Trypsin, Cells, Virus etc.?

What should we do to eradicate mycoplasma presence from our lab and samples?

05 May 2015 906 9 View

Is there any problem if FBS is stored at -70° C instead of -20° C temperature?

If FBS is stored at -70° C is there any chance that FBS property could be changed and causes any problems to VERO cells after passage?

05 May 2015 1,795 2 View

What factors can cause false-negative in hemagglutinin (HA) assay

we are getting false negative in virus samples. Please share your ideas. Tried 0.5% chicken RBC, 0.75% guinea pig RBC and 0.4% chicken+guinea pig RBC. Also tried with young chicken blood. every...

01 January 1970 3,402 10 View