Abdul Khaliq

5 Questions 3 Answers 0 Followers

Questions related from Abdul Khaliq

Kindly read the question and attached chromatogram. How to separate peaks in HPLC?

I have methanol extract of a plant, Flavonoids are expected in it. I run reverse phase HPLS, C18 Luna column, Acetonitrile and 0.1% Formic acid mobile phase. I got some peaks, one of them...

02 February 2020 2,561 3 View

Dry Column Vacuum Chromatography (DCVC) and Vacuum Liquid Chromatography (VLC) are same OR diffrent techniques?

01 January 2020 7,136 2 View

What is the max. conc. of compound 991, which can be used?

I am using ex229 (compound 991), (10 µM), as +ve control for AMPK activation (phosphorylation) in J774 cells (macrophages), but it is not working (Western Blot). It is showing +ve effect on p-ACC...

01 January 2020 7,522 1 View

Does agitation speed affect antibody binding?

Does agitation speed of orbital shaker affect the binding of primary antibodies when we incubate the membranes overnight in cold room? If agitation stops during night, will it affect the results?

11 November 2019 2,503 1 View

How to design method (mobile phase, column) to identify maximum number of compounds in an extract?

I have MeOH plant crude extract, which has shown in-vitro anti-inflammatory activity (I am working on inflammation). I run on HPLC using 0.1 % Formic acid and Acetonitrile and got few peaks. I...

10 October 2019 9,581 5 View