Can you recommend a good review on methods for transcriptome analysis? In our lab we expand human T cells and magnetically seperate cells being positive for our target protein. Now, we want to compare transcription status between these cells and control sets. For this, i'm looking for a good review comparing different transcriptomic techniques (eg. singe cell RNA seq., microarrays, ht-RNA seq etc.) with a special focus on costs, time requirement, advantages and limits. Many thanks and kind regars, Marc
Dear Marc Lucas Appis ,
A review that covers all aspects you mentioned is kind of hard to find. At least, I did not come across a review that tackles the topic of "time requirement". I think that the most complete RNA-seq review I have with me right now is:
(1) Van Den Berge, Koen, et al. RNA sequencing data: hitchhiker's guide to expression analysis. Annu. Rev. Biomed. Data Sci. 2019. 2:139–73;
If this review does not help, let me know, and if I find another one that fits your requirements I'll post here.
Cheers,
Bruno
Thank you Bruno César Feltes , this seems to be close to what i was looking for. If there are no comprehensive reviews on transcriptomics, of course a short list of reviews would be fine too. So thank you very much for your paper recommendation!
Hi colleague
Find the following URL, may help you:
https://www.sciencedirect.com/topics/neuroscience/transcriptome
Regards..
I thank you for your good hint, Prof. Ayad Ghany Ismaeel ! This is a great starting point for finding comparative transcriptomic methods papers.
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