Can anybody please suggest YEM medium composition for Agrobacterium tumefaciens LBA 4404 strain. Is YEB medium suitable for the above strain?
following composition of YEB works very fine
D-Mannitol: 10%
CaCO3: 4 %
K2HPO4: 0.5%
yeast extract: 0,4%
MgSO4.7H2O : 0.2%
NaCl : 0.1 %
distilled water
pH 6.8-7.0
for Agrobacterium you can used Yeast Manitol Broth/agar, or Luria Bertanii for miniprep plasmid
i suggest you using YMB,
YMB compositions are
Yeast extract : 1g/l
K2HPO4 : 0.5 g/l
MgSO4.7H2O : 0.2 g/l
NaCl : 0.1 g/l
D-Mannitol : 10 g/l
distilled water 1 liter
but if you want YEM (do you mean YEM is yeast extract mannitol medium ?, if yes i give you the recipe taken from handbook of microbiological media)
D-Mannitol 10g/l
CaCO3 4 g/l
K2HPO4 0.5 g/l
yeast extract 0,4 g/l
Best Regards
Ridzky
YEB is suitable for above strain and i have used the same good luck
Composition for YEM medium
Mannitol-10.0g/l
Di-potassium orthophosphate-0.5g/l
Magnasium sulphate-0.2g/l
Sodium chloride-0.1g/l
Yeast extract-1.0g/l
Calcium Chloride-3.0g/l
Agar (If applicable)-15.0g/l
pH-6.8-7.0
If u want LB medium composition i will send collected from Micrbiology Laboratory Manual by Dr. S M Reddy and above composition is from same book.
You can use TY medium
yes, YEB is suitable for above LBA4404 strain good luck sir
Media - YEM (YEast extract - Manitol)
YEM: Composition: Mannitol: 10g, yeast extract: 0,5g, K2HPO4: 0.5g, NaCl: 0.2g, MgSO4.7H2O : 0.1g CaCO3: 4 %
pH 7.0
Prepare 16.6% CaCl2 solution.
Autoclave for 20 min at 120 °C
Add 1 ml of the 16.6% CaCl2 solution to 1 liter YEM medium (1:1000)
Let cool to ca. 55 °C before adding antibiotics
Store at room temperature or 4 °C
we are using 10g BactoTMyeast extract, 10 g BactoTM Peptone , 5g sodium chloride for 1 litter volume and adjusted to 7.5 pH. Finally we autoclave and use after cooling or store it at 4°C until use.
please give any suggestion?
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