Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

How often machine learning is used for STEM research nowadays?

I am an undergrad physics student. Nowadays, I observe a tendency among physicists to learn machine learning. Surely, it's highly useful to know ML when you have to deal with tons of data from...

14 January 2021 6,431 4 View

How to segregate electrode-dielectric and dielctric-dielctric interface polarization after Capacitance VS Freq. measurement using impedance analyzer?

After measuring the capacitance vs frequency for my MIM capacitor consisting of alternate layers of TiO2 &Al2O3 , there is a rise in capacitance value of ~ 20-160 nF at 100 hz, starting to...

17 December 2020 7,786 3 View

Error encountered in executing MCE ?

In Terrset, an error encountered in executing Spatial Decision Modeller (MCE). During execution or run the MCE a massage come on the screen that reflects "Your columns and rows are not same" , I...

07 December 2020 1,542 1 View

I want to synthesize a GUV. Can you tell the lipid composition and ratio that I should use that will mimic the biological cell/endosomal membrane?

Can you please provide me the reference

17 November 2020 6,128 2 View

Can anyone suggest suitable RNA immunoprecipitation (RIP) protocol from cell?

I would like to see RNA-protein interaction following transcription process inside the cell. I am wondering if there is any protocol similar to protein co-immunoprecipitation,which can bind the...

27 October 2020 2,940 3 View

How to do extract Urban Heat Island separately from LANDSAT image for each land classes?

I have already been read many articles and found some methods but those are not cleared to me. Can you please suggest me how to generate different LST map of each land classes using ArcGIS? Or...

25 October 2020 5,978 5 View

Would anyone please suggest any book/guideline on Agent-Based-Models that address human migration dynamics?

I would like to assess how the reasons of migration varies across type of environmental migrants, and therefore, how it could be useful for modelling adaptation strategies for the future migrants.

04 October 2020 5,435 7 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View