What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Life of satisfaction scale?

Hai sir. I'm a student who are doing my final year project for my degree. Can i get the questionnaire of life of satisfaction scale for my final year project? Please do help me sir.Thank you sir.

09 November 2020 308 2 View

How to go about designing primers?

I am working on gene sequencing for an organism that has little to none sequencing done. I have acquired the homologous sequence for that particular gene across three species. I would like to know...

24 August 2020 6,379 3 View

I have RNA sequencing data which i would like to analyse for expressions, which software should i use to study the gene expression?

this is my first time doing bioinformatics, so a user friendly software would really be good. Thank you.

08 June 2020 6,737 15 View

No compression (tension only) truss elements in LS-DYNA for modelling ligaments?

Hi, I am trying to model ligaments for a spine model in LS-DYNA using truss elements. I was wondering if anyone could tell me how to define a linear elastic material for the trusses that only...

15 January 2019 3,214 3 View

Hello everyone, I am planning to construct a tool on "parental expectation" from the school students' perspective. please provide your suggestion?

Provide your suggestions on how to do it and what kind of expectations parents have (such as educational, social). Also, I would be grateful if anyone explain on research methodology part on tool...

08 February 2018 5,601 5 View

How to calculate the initial head space pressure required to keep water in liquid state?

26 July 2017 421 3 View

How to prepare MnCl3?

Good Evening! At present I am synthesizing some manganese doped nanoparticles. For that I am in need of MnCl3, but it is not available commercially. Will anyone can help me to synthesis MnCl3. I...

29 March 2017 8,080 8 View

Which state of matter exists first in universe?

There is four state of matter exists in nature; solid, liquid, gas and plasma. The origin of everything is Big bang; I need to know which state of matter exists first in the universe.

09 November 2016 5,463 22 View

How to construct a moving average control chart when the target value and or the Sigma values are not available?

Please refer the Section 9.3 of D C Montgomery (6th edition, page 428). He has given an example to construct an unweighted moving average control chart. Suppose that the target value and the Sigma...

03 July 2016 6,548 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

The procedure compute?

02 March 2021 5,204 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View