How can I prevent Ngn2 neuron-astrocyte co-cultures from peel off the coverslip?

Hello everyone, I've been co-culturing hiPSC-derived Ngn2 neurons with human fetal astrocytes, and at the time of grabbing the coverslip from the plate (24 wp) the cells peel off the coverslip. I...

03 September 2020 2,947 1 View

Any suitable DARPP-32(Thr34) antibody?

Does anyone know about any supplier for DARPP-32(Thr34) antibodies that work well in immunofluorescence? Specifically to prove in mouse and rat tissue.

01 February 2020 4,049 1 View

Does anyone knows why output parameter is not working in this PyQgis script?

The problem must be the definition of the output string, because I tried the same code but writing a route directly on the parameters list and it worked. This is the code I wrote: import...

07 August 2019 6,108 4 View

Problem confirming discriminant validity (SmartPLS). Could you help me?

14 September 2016 4,923 9 View

Does UV-crosslink also work between proteins? And how is it possible to "reverse" it?

UV-crosslink is widely used to bind together proteins and RNA for co-isolation. Does it works also to connect proteins with other proteins? Is it possible to "reverse" such crosslink? For example,...

27 June 2016 7,546 3 View

How to reorder factors on plotMeans using R commander?

I did a plotMeans to visualize the results of Kruskal-Wallis test between groups. But I need to reorder the levels of a factor because the default order is alphabetical and I want to show it in...

19 January 2016 949 3 View

How to reorder factors on plotMeans using R commander?

I did a plotMeans to visualize the results of Kruskal-Wallis test between groups. But I need to reorder the levels of a factor because the default order is alphabetical and I want to show it in...

31 December 2015 404 3 View

How to interpret vs. test in MCA analysis run by FactoMineR in R commander?

I did a MCA analysis using FactoMineR. I know how to interpret cos2, contributions and coordinates, but I don't know how values of v.test should be interpreted. Thank you

09 October 2015 8,383 3 View

Activated charcoal to remove pigments during DNA extraction

Has anyone ever used activated charcoal to remove pigments during DNA extraction? In which form should it be added? Should it be sterilized? Is efficiency dependent on temperature, pH and time?...

06 July 2012 4,191 8 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

What is the most important parameter of a research work, the impact factor or the h index?

The impact factor is a measure of the importance of a journal based on the number of citations received in a year. ... Articles published in a journal with a high impact factor do not all receive...

24 February 2021 7,481 2 View

What is the original citation for the commonly used activity factors for converting basal metabolic rate to total caloric need?

I see the same activity factor (multipliers) in research papers and websites, but I cannot find where they come from. Does anyone know where these originated? I am looking for an original...

18 February 2021 7,635 2 View

Which and How to use teaching strategies and procedures to effectively integrate the four skills while teaching English Language Online?

As an inexperienced teacher in the field of online teaching, I have the fear of unconsciously ignoring one of the skills while focusing on not ignoring the other! Therefore, I wish to make use of...

09 February 2021 5,101 4 View

Why the FIGURE inserted through endnote 20 is not showing in word?

I wanted to insert the FIGURE files through endnote 20, but the image is not showing in the two-column IEEE Journal template. However, the image inserting position is marked as (Figure 1), (Figure...

05 February 2021 8,167 1 View

How do I correct an inaccurate citation?

I was asked to verify a citation: Drugs and galencials, by D. C. Garratt, B.Sc., Ph.D (Lond.),... The author's first name is Emerson and the last name is Beeler. It was incorrectly cited as...

03 February 2021 5,272 3 View