I'm running gel electrophoresis for bacteria DNA. I used GeneRuler High Range DNA Ladder, ready-to-use. However, when I run gel electrophoresis, the DNA ladder isn't separating well. According to protocol, it should separate in 1.5hours if ran at 3V/cm.
I ran it at 60V, 2hours, 0.4% agarose.
The first lane is the ladder. Can anyone help me with this?
I think you need use more % of agarose. I do the gel with 1% agarose and when i need more separating i use 2 % of agarose gel. I use this to mouse DNA. I hope i can help you.
Hello Lim,
my first impression from your photo is that there is too much marker. So, you could dilute your marker and see which dilution works best.
The seconed thing is that you should use TAE buffer instead of TBE buffer for separating large fragments (maybe you did but you didn´t mention it).
best wishes
Dirk
Hello
i think you need to increase the agarose concentration up to 1% ,
also check your buffer >
regards
Hi,
You need to increase ( ran it at 80-100V, 1hour), and increase the agarose concentration up to 1.5%.
best wishes.
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