What does this SAED image of nanoparticle sample signify?

I couldn't make any inference from this image. please help

30 August 2020 8,442 4 View

I got diffused bands in western blott? What could be the reason?

I ran western blot, for bax (20kd), 15%gel, 100v , then transfer overnight 40V 4 degree, I am attaching photos ( ponceu/ chemiluminiscence)kindly help me out , why bands get diffused-

04 February 2020 4,493 3 View

Why sometimes mdamb231 cells grows in patterns like rows in-between in few places in flask??

I am new in cell culture techniques, I am maintaing 231mdamb cell lines right now, cells seems healthy and growing well, but I observe in one flask cells are growing in rows inbetween scatterted...

13 October 2019 2,509 4 View

What is the reason behind the different values of the band gap of the same material calculated from two different techniques?

I have calculated band gap of cerium oxide using UV-vis absorption spectra and PL emission spectra. The values of band gaps calculated by both technique differ by ~ 0.7eV. What can be the possible...

24 September 2019 4,171 5 View

i want to isolate proteins( related to apoptosis) from breast cancer cells for western blot, how to make cell lysate for maximum protein yield???

I am new to western bloting, i need to isolate protein ( bcl2/ bax/ nf kappa b/ caspase) by western blotting from breast cancer cells, i will use ripa buffer with protease inhibitor cocktail , do...

09 September 2019 2,550 2 View

Can we investigate Ic50 of drug without knowing its drug loading capacity in case of drug prepared with polymer?

I have prepared arsenic loaded nanoparticles now m looking forward 4 anticancer properties of this drug but i have nt calculated drug loading capacity yet..can i go straight for mtt assay to know...

18 December 2018 1,039 4 View

If i have protocols for tissue samples (kidney/liver) viz gsh,SOD,protein can i use them for cell culture(breast cancer cells)???

I have worked on invivo toxicity of liver kidney now i m shifting towards toxicity in mammalian cancer cell line. Can i use protocols i already have?

31 May 2018 5,598 4 View

IMPACT FACTOR of the journal?

Hello Everyone..!! Please tell me the impact factor of " international journal of applied engineering research" ..

14 April 2018 3,629 4 View

How can I keep the iron in its ferrous state in the reaction medium?

04 July 2017 6,495 2 View

ALCOA is a standard used by FDA and WHO for data integrity can this be used generically in IT sector as well for maintaining the data integrity?

ALCOA is a compliance used in life sciences and pharmaceuticals for maintaining the integrity of the data. i am thinking of using this standard in IT sector for maintaining the integrity of the...

19 October 2016 1,245 1 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

VIF very high, but correlation is low and coefficient is significant. Which one whould I choose?

Hello. I have a question whether if my coefficients (statistically significant) would be valid when VIFs are really high. In my model, there are independent variables(IV), moderating variables...

26 February 2021 956 13 View

Treating Intermediate Variable in Linear Regression Analysis in SPSS?

While performing the Linear regression analysis in SPSS version 26.0.0. my intermediate/mediation variable goes to the excluded section. Please share your valuable suggestion "How to restrain the...

25 February 2021 4,591 5 View

Cox regression baseline hazard?

Dear colleagues, I would like to make a mathematical equation of Cox regression model, however I am stuck with baseline hazard. I clearly understand interpretation of coefficients, but I can't...

24 February 2021 6,207 3 View

How to run raise regression in R?

if anybody know how to run raise regression in r, and raise parameter selection, please help me

24 February 2021 8,199 1 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View