Looking for Turbidity of a solution defined as an absorbance value >0.015OD, which is measured using UV equipment. Wavelength used in most of the incompatibility papers is 546nm, irrespective of the analyte involved. Any suggestions as to why this wavelength is picked? Or any guidance to a research paper which might have a possible explanation?
Hello Megha,
I'm not familiar with turbidity of solutions and its measuring techniques, but does UV in your question stands for ultraviolet (radiation)? A wavelength of 546 nm is far away from the UV range, which ranges from 100 nm to 400 nm. 546 nm is green visible light. With pure UV (ultraviolet) measuring equipment you will not be able to measure the absorbance (transmittance?) of green light.... Or have I misunderstood that?
By UV- i meant i am using UV-spectrophotometer for measuring absorbance at wavelength 546nm for looking turbidity of the sample.
Ok. And what kind of a UV-spectrophotometer do you use? Do you know the manufactorer, typ, maybe the sensitivity and wavelength range?
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Pharmaceuticals
- Drugs