Megha Krishnakumar

5 Questions 4 Answers 0 Followers

Questions related from Megha Krishnakumar

Any suggestions on how to talk about high peak area associated high recovery value in HPLC?

Concentration of sample was within the calibration curve range, seeing couple of outliers with an increased peak area and subsequent increase in recovery value in HPLC data results of >105%-250%....

05 November 2019 593 1 View

Any Suggestions to rectify negative intercept in HPLC?

Drug is Sodium citrate, diluting drug in de-ionized water. Using a C18 column. the concentration range of 1000-3000microgram to lower range of 25-300microgram. Thanks!

20 September 2019 7,584 3 View

What would be the mobile phase for quantifying sodium citrate using HPLC method?

I have to determine concentration of sodium citrate using HPLC method. Previous literature's used potassium phosphate buffer of pH 2.5 as mobile phase (isocratic). As there is risk of clogging the...

01 August 2019 1,288 5 View

Why wavelength 546nm (UV) is chosen to check incompatibility of 2 drug solutions?

Looking for Turbidity of a solution defined as an absorbance value >0.015OD, which is measured using UV equipment. Wavelength used in most of the incompatibility papers is 546nm, irrespective...

31 May 2019 5,891 3 View

UV spectrophotometer of Ethanol/ trisodium citrate dihydrate?

I am working to find incompatibility or precipitation point at different concentrations of ethanol from 10% to 50% with 4% IV bag Trisodium citrate. I am looking to find turbidity i.e Absorbance...

01 May 2019 3,825 2 View