Problems with library virus generation?

Dear all I cloned an sgRNA library into a pLenti plasmid backbone (puromycin selection marker) by golden gate ligation and transformation. However I cannot produce any virus from the library...

25 October 2020 3,820 2 View

How can I create a Venn diagram using SPSS?

I have 5 sets with 200 variables.

14 October 2020 9,681 4 View

Transfection of HEK293T cells with linear dsDNA?

Does anyone have experience transfecting or electroporating linear dsDNA into HEK cells? If yes what is the size limit, what size have you tried and worked. In principle, I want to do HDR, and...

25 March 2020 5,649 3 View

How the Aluminium Oxide layer on Battery Terminals affect the performance?

Hello Everyone, I am currently optimizing the production process for the battery packs. I would like to know how the great the increase in contact resistance with an aluminium oxide layer on the...

21 May 2019 2,782 3 View

Why DCIR value changes in the Battery?

I am using LiFePO4 battery, I measured DCIR using the discharge pulse. I found that the measured value of DCIR changes with respect to both pulse current and SOC. I provided adequate rest period...

28 January 2019 5,227 4 View

How to measure the energy consumption by DC system?

I am designing Battery pack for a DC system. In order to find out the capacity required for the system, I need to calculate the energy requirement (Variable load, Idle condition). Since the...

10 September 2018 9,766 8 View

How to test BMS?

I would like to know how a BMS from a different Manufacturer can be tested? I want to make sure whether the BMS works as per our requirement and also ensure its operation as per the data...

08 May 2018 5,204 3 View

What is Battery Size Factor? when it should be used?

Hello Everyone, I would like to know what is Battery Size Factor? How it is related to Battery Testing and when it should be considered? Thanks in advance

29 April 2018 7,871 4 View

How to find out diffusion coefficient using warburg impedance?

11 September 2017 1,196 13 View

How to infer the data of Oscillation Amplitude Sweep?

04 September 2017 4,820 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View