when I measured the total flavonoids and total polyphenols of my 15 day old plants (Vigna unguiculata), I found that the amount of flavonoids is greater than the amount of polyphenols, and to my knowledge flavonoids are polyphenols. so please explain
While it is not clear how you experimented, there might be a need to check the suitability of your calibration graphs, verify/validate your method before actual measurements, and make use of standards eg phenol and quercetin, use of reagent blanks etc. Sometimes the answer lies in quality control. I am assuming you were using a UV-Vis spectrophotometer. More information from your experiment could have shaded more light.
Nature of sample and sample preparation can also result in such errors
Dear Bahdja yasmine Agrane,
The reason of this result is complex, which may related to your solvent, standards and methdology.
You could try my methods for TPC and TFC which is published recently in Article Evaluation of the chemical composition and nutritional poten...
Hope it could help you.
Best regards,
Weihao
Dear Bahdja Yasmine Agrane,
we need more information on the methodology adopted.
But, if already the standards used for the calibration of the polyphenol and flavonoid contents are different, then it can happen that the flavonoid content is higher than that of the total polyphenols.
Best regards.
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