The NIF system is the first discovery of Fe-S biosynthesis pathway in Azotobacter vinelandii, it is known to function in the specialized assembly of Fe-S clusters for the nitrogenase enzyme in nitrogen fixing organisms .
Hello everyone, I'm in the initial stages of conducting a study involving the ddPCR (Bio-Rad QX200, QX600) and I ran into some problems involving recovery % calculations using direct...
02 July 2024 6,011 2 View
Is the zirconium isotope of geological sample fractionated when passing through the column, and how to determine whether this fractionation is or not?
26 May 2024 6,721 2 View
In C++, why is the constructor of a virtual base class called first, while the object of the virtual base class is placed last? What are the advantages of doing this?
07 May 2024 1,583 1 View
Just like the stray cat protection organizations on campus, they are spontaneous and not organized by the school. They provide a series of services for kittens in life and death. And the source of...
11 April 2024 4,944 0 View
We are using pMSCV for transit expression of a protein.: 1. The gene was cloned in (between XhoI/EcoRI). 2. Transfection with lipofectmin 3000 to 293t cell. 3. After 48 hrs, GFP can be...
04 April 2024 8,350 4 View
In the latest CAZy sequence list (http://www.cazy.org/), over 3,600,000 sequecnes have NCBI accession and they can be easily accessed. However, the remaining 550,000 sequences only have a jgi...
13 March 2024 2,439 1 View
Computer Systems Science and Engineering ISSN:0267-6192(print) Publish within 4 months Low APC: 600 USD(Review paper full waiver) Send me DM for more info
31 January 2024 5,463 1 View
In 1D random strustures, the Anderson localization is an important phonomenon. Localization length is a key parameter to show the localization characteristic. How to calculate the localization...
28 December 2023 3,999 1 View
Hi! I have questions to consult with scholars. We consider a multiuser MISO downlink communication system where an IRS equipped with a UPA ( with M reflecting elements) is deployed to enhance the...
08 December 2023 6,883 4 View
Hi! I have questions to consult with scholars. We consider a multiuser MISO downlink communication system where an IRS equipped with a UPA ( with M reflecting elements) is deployed to enhance...
07 December 2023 7,563 2 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.
26 July 2024 1,469 1 View
Hello, I am trying to clone 1334 bp insert into 6.1 kb vector using HiFi DNA assmebly/Gibson assembly kit. I am taking 100ng of vector and 1:5 molar ratio of vector: insert and 4 hrs of...
27 June 2024 9,652 2 View
To do gibson assembly cloning can I use DH5alpha strain of E. coli. for transformation?
20 June 2024 774 1 View
I am trying to do gibson assembly cloning. I have two fragments for gibson assembly cloning (plasmid backbone and insert). After gibson assembly reaction I have transformed this in E. coli....
19 June 2024 6,404 4 View
I have to join tungsten wire to metallic plate with that adhesive and entire assembly has to be heated upto 600C in ultra high vacuum. I know about zirconia paste but it is not readily available...
18 June 2024 3,092 3 View
Dear cloning community, Is it possible to use Overhangs with large Tm difference with NEB Builder HiFi DNA assembly? 5´ 26 bp Tm 57ºC 3´ 24 bp Tm 72ºC Unfortunately, extending the 5´ doesn't...
17 June 2024 8,552 2 View
I have an assembly of parts. I want to reduce the number of modes of the individual parts by CMS.
11 June 2024 2,966 0 View
In my undergraduate research project, I require an integrative plasmid containing GFP with an antibiotic resistance that can't be kanamycin. In our lab, all of our integrative-GFP-containing...
14 May 2024 9,861 3 View
I would like to do genome assembly for the bacteria isolate from the environment. Can any provide me the information or any tutorial? It would be helpful! thanks!
18 April 2024 1,894 12 View