The NIF system is the first discovery of Fe-S biosynthesis pathway in Azotobacter vinelandii, it is known to function in the specialized assembly of Fe-S clusters for the nitrogenase enzyme in nitrogen fixing organisms .
Selfconsistent noncollinear magnetic calculations can be done in VASP. I am wondering how VASP update the spin directions at each local site. For molecular dynamics, VASP search the next...
16 January 2021 1,398 3 View
I want to ligate a linear 1.7kbp plasmid with the EcoRI sites at two sides, but when I use the quick ligase kit from NEB to test the ligation efficiency it is hard to ligate it. Is there anyone...
08 December 2020 3,442 3 View
Recently I have been involved in this problem: After expressed by adding IPTG, lysed with B-PER, and resolved with Buffer B (8M urea), incubate with Ni-NTA beads, everything looks fine. When I...
21 November 2020 7,416 3 View
Hi, I was doing the Abaqus explicit model for a plain woven fabric where two types of yarns(PU&PA) were set as the weft and one type of yarn(PA) was set as the warp. When the load applied to the...
10 September 2020 1,164 2 View
I am graduate from other major, and now working in computer science and technology, my roomates are mostly researching on image recognition. I want to switch to it and learn how to cooperate with...
21 August 2020 2,888 9 View
I differentiated THP-1 cells to macrophages using PMA(100ng/ml) and then used lentivirus to deliver shRNA to knockdown my target genes. Unfortunately, it was too difficult to be transfected...
16 August 2020 5,073 3 View
I want to rotate the 1,2 components of OBS to the R, T components, but the SAC command running results show that the two components are not horizontal. Could anyone can teach me how to correct...
06 July 2020 2,536 3 View
People are told that paracetamol or ibuprofen can only be used to treat the symptoms of COVID-19, but we think that taking paracetamol and vitamin C or ibuprofen and vitamin C every day is a...
01 July 2020 3,058 4 View
when I run ORCA, I got an error as follows: ORCA finished by error termination in GTOInt Calling Command: mpiexec -np 4 D:\ORCA421\orca_gtoint_mpi.exe na20.int.tmp na20 [file...
08 May 2020 5,444 3 View
Dear Colleague, I would like to find some original literatures studying on aethetic feeling. Many literatures have focused on how to possess this capability, like experiential learning. But it...
02 May 2020 6,966 3 View
I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...
16 February 2021 8,872 2 View
I'm trying to do a simulation between the Maxilla and the Mandible I converted the Dicom file from the CT scan to a CAD module and module the articular discs via 3d software. I have two options...
08 February 2021 8,731 2 View
The continuity boundary condition is automatically created for a shared (common) surface boundary but I want to have it for an edge. Please help me out. In image 8 is the base of the cylinder and...
05 February 2021 3,459 2 View
Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...
03 February 2021 2,032 3 View
Hello! I'm trying to assemble some plasmid vectors that contain two repetitive regions (with around 500 and 250 bp) and it is being hard to get the complete sequence. If someone knows a better...
03 February 2021 6,379 3 View
The bacterial NRPs (nonribosomal polypeptides) are famous as clinical drugs such as antibiotics, but is there other native function of these NRPs of bacteria except antimicrobial, because their...
01 February 2021 4,280 1 View
Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...
01 February 2021 6,756 4 View
Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...
29 January 2021 1,406 2 View
I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...
29 January 2021 7,764 6 View
I am trying to import an Abaqus .inp file to Ansys but it does not import the whole assembly but only imports a single part. How to solve this.
17 January 2021 5,450 3 View