why the Exonuclease activity was inhibited with the increase of magnesium ion concentration?

05 December 2021 0 9K Report

some kinds of exonuclease need MgCl2 to degrade RNA, but why the Exonuclease activity was inhibited with the increase of magnesium ion concentration? the functional RNA folding needs MgCl2, low concentration of MgCl2 will lead to RNA unfolding? if we want to determine the structure of the exonuclease and functional RNA complex structure, how we can choose the concentration of the MgCl2?

Badges
Science topic

More Wendy Jiang's questions See All

Has anyone tried freeze flow samples in -80C freezer or liquid nitrogen before processing?

Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...

15 July 2024 9,975 1 View

When Streptomyces undergoes conjugation transfer, it is always contaminated by a type of yellow Pseudomonas. What could be the reason for this?

07 July 2024 5,480 2 View

If the weghts of convolution kernel in CNN(convolution neural network) are pre-designable, or weights must be initialized and updated while trainning?

Are the weights of convolution kernel in CNN(convolution neural network) pre-desighable before trainning?

20 June 2024 6,501 6 View

In CNN, is the feature map obtained randomly by convolution kernel?

In CNN(convolution neural network), can the feature map obtained determinately by a random initialization convolution kernel? if not, how to decide the weights in convolution kernel to obtain the...

20 June 2024 6,418 6 View

How do the Maxent use to prediction the distribution of the cave fishes in Karst area?

As the known, there were the most richness species diversity of the cave animals, especially for the cave fishes, because the underground river sytems. But there were the diffculties exist also,...

14 May 2024 9,131 0 View

Help me find the full text. I can only find the summary part in many databases, and I can't see the specific content. The author did not reply to me ?

Resveratrol supplementation or diet intervention effects on heart-liver axis in prediabetic female rats submitted to high-fat-high-sucrose diet

08 May 2024 9,891 1 View

Help me find the PDF, not only the abstract?

Resveratrol supplementation or diet intervention effects on heart-liver axis in prediabetic female rats submitted to high-fat-high-sucrose diet

05 May 2024 8,818 2 View

Any publicly accessible human bivalent gene database?

There was a database from a university in China, but it seems no longer accessible to the public. Are there any other database to search for bivalent genes in human? Thanks.

24 April 2024 976 0 View

The components of a pancreatic exocrine organoid using mice pancreas?

I am working on pancreatic cancer. Now, I am trying to generate a pancreatic exocrine organoids using my KPC mice. But after I checking the protocol, PancreaCult™ Pancreatic Organoid Growth Medium...

20 April 2024 3,879 1 View

Please help. What is the name of the lock acquired before entering the soft interrupt in the Linux source code?

My experimental environment is Linux6.1, and the real-time patch preempt_rt has been applied to improve the real-time performance of the network. I checked the relevant papers and saw a lock...

18 April 2024 8,721 3 View

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

A twin-shaft kiln or a rotary kiln for lime production 1 class? if compressive strength of limestone is low 60 MPa?

My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...

18 July 2024 1,390 2 View

Why am I seeing only one initial maximum in torque-time curves for the fusion of PVC in a Brabender?

We're studying the stability and fusion behavior of multiple PVC compounds in a Brabender. After a few trials, we set the temperature at 195 °C and the rotor speed at 70 rpm. Samples differ in...

12 July 2024 2,768 1 View

Can an Ischemic Buffer Alone Effectively Mimic Hypoxia in an In Vitro Cardiac Cell Hypoxia-Reoxygenation Model?

I am working on an in-vitro model of hypoxia-reoxygenation (H/R) in cardiac cells (H9c2). Due to the unavailability of a hypoxic incubator, I am exploring alternative methods to create hypoxic...

30 June 2024 1,234 2 View

Root Phenotypes in Arabidopsis Col-0 Under Phosphate Deficiency Conditions not showing up??

Introduction: I am conducting an experiment to investigate the root phenotypes of Arabidopsis Col-0 plants grown under phosphate deficiency conditions. Here, I describe my current methodology and...

19 June 2024 8,774 3 View

Can someone introduce an article about the convergence of the temperature parameter for the SPH part in Abaqus software?

For the article, I need some references for the subject of checking the convergence of the temperature field parameter for the SPH part in Abacus, and I want to study this case with a few meshes...

13 June 2024 2,504 0 View

Is something wrong with my setup that I cannot elicit a response from field EPSP?

Hi all, I have been attempting to do field EPSP but I am not able to elicit a response. I am using a thin walled electrode for the recording electrode at 1.5mOhm resistance. I have filled the...

10 June 2024 2,418 0 View

What could dissolve MgO nanoparticles over time?

I have a set-up were I have a bottle with water (DI water, 0.8uS/cm). To that bottle I have added some MgO nanopowder, at a concentration of ~ 0.08mg/ml. It appears that the white powder is...

04 June 2024 9,789 4 View

May i use mgcl2 or cucl2 or bivalent salt in place of calcium chloride for crosslinking the membrane consisting sodium alginate. which is better?

i am working on self heating membrane preparation. so for crosslinking of membrane, may i use copper chloride, or magnesium chloride or any bi valent cation salt to crosslink it? and which one...

29 May 2024 2,474 0 View

DNA foot-printing buffer components? What are their roles?

Hi, I am learning to perform footprinting experiment. In the protocol I have there are the following components of the reaction buffer: HEPES buffer (7.8 pH), glycerol, BSA, DTT, KCl, MgCl2,...

28 May 2024 1,317 0 View