The formaldehyde gel electrophoresis was used to check the RNA integrity. The standard protocol was used but the bands are visible on the backside of the well.
What is the problem here? How should i correct it?
The most common reasons for samples running the wrong direction are
1 the electrodes are connected the wrong way round.
2 The pH of the buffer is very wrong and everything is incorrctly charged and runs the wrong way. If your dye has no orange G dye in it then the orange colour of the dye in the top figure suggests that the buffer is too acidic and some of the blue dye has changed colour in much the same way that litmus changes colour in acidic solutions. It is possible that the buffer is too weak and there is no buffering at the ends of the gel which are becoming acidic and basic as the ions move to their expected ends
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