As usual i am checking my Agrobacterium strains with cabbage, carrot and potato. But in potato i am seeing it turns black color. Is it because of oxidation or some thing else? Kindly help me in this regard.
Thank you
Probably the phenolic compound caused that. I saw that phenomena on cotton genetic transformation, around the explants. But I don't know about potato.
Hello Anil,
Which part(s) of potato you are infecting by Agrobacterium and trying to transform ? Potato infected explants may respond differently by secreting some phenolic compounds as a part of defense mechanisms
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4086400/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3866890/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063549/
Hi Anil,
[I posted another answer earlier, but I did not see it. So I am re-posting it and add some more info]
1. I think that it probably caused by phenolic compounds. I saw this same phenomenon on cotton transformation. It became dark around the cut explants. But, I don't have experience on potato.
2. By the way, what material did you use for transformation?
3. I attach a 'potato transformation slide' for you. They use leaves. You can estimate the size/age of the leaves (with the petri-dish) they used for genetic transformation. You might be able to get an idea of how other researchers perform potato transformation.
Respected Yuan Sir
Good Evening! Hope all your health is good. May be Phenolic compounds are oxidizing in my case. I am using direct potato ( Cut into small pieces) and giving infection for 20 min with Agrobacterium strains.(Half Strength MS Media)
But Interesting one is i am doing my experiment to just check weather the strains are in good condition along with my seed ( that is i am giving infection to seed also along with potato) But i am not finding color change in my seed. Is seed not release phenolics into media?
But your attached photos and answers have always i am getting lot of information and i am checking in everytime for your answer for more information.
I highly obliged to you sir
Thank you for your support
Respected Abdullah Sir
Thank you for attaching articles for more information. In my case i am using direct potato ( Cut into small pieces) and can you please let me know how many days it will need to understand weather respected gene is integrated into our target system (potato).
Thank you for your support
Its better to use in-vitro maintained shoots for explants. we always use internodal cuttings of 5mm and routinely transform using Agro. Potato tubers have lot of bacteria in them and could lead to contamination problem.
Thank you Purushothama Sir
Yes ! You are absolutely true, Is Contamination Inhibits HR Disease?
Thank you
Anil,
potato tubers are very sensitive to oxidative stress, especially at high temperature - they turn black. Agrobacterium can cause production of superoxide anions (O2°−) in the infected tissue of plants/ See http://onlinelibrary.wiley.com/doi/10.1046/j.1365-313X.1996.10030545.x/pdf
this link is useful
https://www.banglajol.info/index.php/AGRIC/article/.../11068/8095
regards
- Badges
- Science topic
- Similar topics
- Philosophy
- Moral Philosophy
- Free Will