I have been reading up on the TGR5 receptor and how it could be used in treatment of various aspects of metabolic syndrome, however, a crystal structure has not yet been found and through my reading I cannot find why, so I was wondering if anybody knew and what studies could be done in order to solve it?
the reason for no structures is that it is a membrane protein and currently there are only a few hundred membrane protein structures compared to several thousand soluble proteins. Also as a class of MP the GPCRs are particularly difficult probably because they are less stable in detergent than other membrane proteins, In fact there have been consortia set up to look at the structural genomics of GPCRs because they are recognized as difficult structures to obtain, See Lundstrom in Trends Biotechnol, 2005, 23(2), 103.
Dear Ritesh,
I suggest homology modelling technique to solve your problem.
for more information about homology modelling I attached a pdf plus a link for modelling software.
best
HAH
http://salilab.org/modeller/
Many thanks for the quick replies, will take a look at all information here
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