Its boiling point is 247 deg.C. It will be hard to move it to headspace phase at high rate.

In what temperature are you thermostatting your sample (and how long)?

You can try to increase the temp or use additionall techniques like SPME od SDME coupled with headspace.

regards,

GB

Grzegorz Boczkaj I am doing it at 250 deg. C .

But i anyway managed to find it with just GC-MS.

Thank you.

Regards,

Meg

Nicotine has good MS response. But why do you do headspace analysis? I suggest direct injection (e.g. solvent extracts after LLE/DLLME etc.) of Your sample using split/splitless injection mode (of course it depends on Your matrix).

Best regards - Mateusz