Chitosan is not soluble above ~ pH 6. so it is not water soluble. chitosan can dissolve in diluted acidic solutions so you can try 1% acidic acid, 1M HCl... stir for sufficient time (not in seconds, you may need minutes). For 1% chitosan in 1% acidic acid it may need 1-2 h to dissolve.

Usually nanoparticles do not totally get dissolved in any solutions they usually settle down.But still you can give a try using above method.

Hope it will help you,

Regards,

Khandavalli

The molecular weight of chitosan you used, the concentration of chitosan solution before addition of TPP, the amount of utilized TPP and the way of TPP addition as dropwise can be considered as important parameters affecting nanoparticles specifications.

I hope this paper will help you Article Preparation and Characterization of Water-Soluble Chitosan N...

Thank you for your answer.

I have produced all the chitosan and TPP, BSA of different concentrations. Nevertheless, they are not all dissolved. Drug release assay and cytotoxicity test should be conducted. I do not know what to do. Could you give me some advice?

Khandavalli Ashwini

Alireza Naderi Sohi

Ali Alsuraifi

Chitosan is soluble in acidic conditions - in solution the free amino groups on its polymeric chains can protonate, giving it a positive charge. Chitosan nanoparticles can be formed by incorporating a polyanion such as tripolyphosphate (TPP) into a chitosan solution under constant stirring.

Dear researcher,

You can use weak acidic solution (at pH=6 to 6.5) in order to protonate the surface functional groups of chitosan and change or increase solubility of this NPs in different concentration of acidic solution, easily.

thank you for the reply. But the question seems to be wrong. The chitosan was well dissolved and became a nanoparticle. I want to elute the BSA contained in these nanoparticles, but that does not work, so I ask again.

Asghar Narmani Seyyed Soheil Aghaei Ali Alsuraifi Alireza Naderi Sohi

Khandavalli Ashwini

Chitosan is not soluble above pH 6. so it is not water soluble. chitosan can dissolve in diluted acetic acid solutions so you can try 1% acetic acid.

Pleases see below:

https://www.hindawi.com/journals/jnm/2010/898910/

What is primary purposes of your study ? Would u like to improve water solubilty of BSA only, or also to achieve controled release system ?

You don't need to expect chitosan to dissolve completely if your study was to provided controled release system, because chistosan is primary carrier on this system. where if the chitosan are dissolved, the burst release of BSA will be occured. You don't have to worry if you saw the chitosan are not dissolve on the three of medium pH 7.4, 5.0 and 1.2, but you have to measurement how much the BSA are released per interval times.

Actually i had do the same thing a few weeks ago with the different drug payload, and the results demonstrated a sustained release system.

I feel you should try re-suspending lyophilized CHNPs in acidic water. Moreover, while lyophilizing CHNPs, use of cryoprotectants like Sucrose, lactose, mannitol, and polyethylene glycol may be helpful. If this doesn't work then perform your BSA release assay without lyophilizing CHNPs.

Good luck.

Hi

I'm working on encapsulation of BSA in poloxamer 407 NPs as a carrier but I couldn't release BSA

what is the best method for the release of BSA from my micelles?