@ steingrimur , hi ...I am working on cysteine endopeptidases and metalloproteinases..using Gelatin SDS PAGE followed by incubating gel in renaturation buffer containing Triton-x and then in developing buffer for 16 hrs..after that staining using CBB R 250 and then destaining...
Hi Arvinder Singh . Cys proteinases are readily oxidized and might not show activity in gelatin gels. Metalloproteinases should be visible on zymograms if there are no chelators present, according to this reference.
Article Identification and Purification of Metalloprotease from Dry ...