We have been using an anaerobic jar with an active catalyst and a microaerophilic gas generator pack to culture the H. pylori strain (NCTC 11639) from ATCC on ATCC Medium 260, which is Tryptic Soy Medium with 5% Defibrinated Sheep Blood agar. Unfortunately, our bacterial strain has not grown, even after waiting for 4 to 7 days at 37°C. Could you please suggest any other culture media or potential issues that could be hindering growth?
You can try BHI (brain heart infusion) medium. Also please see Article Grow of Helicobacter pylori in various liquid and plating media
Hello, most studies have used Brucella agar or Columbia agar as the agar base. An amount of 7 to 10% blood improves the growth of H. pylori. Horse blood may also improve the growth of H. pylori as compared to sheep blood.
Karen A. Darbinyan So what do you think , The Tryptic Soy Medium with 5% Defibrinated Sheep Blood agar is not good for this strain..?
I used to add fetal calf serum 20% to Brucella or Columbia blood agar and H pylori grew so well.
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology