Generally Gram negative bacteria are found to be resistant to nisin. So anybody please explain the mechanism behind it. in the other way Gram positive bacteria are sensitive to nisin. How the functional biomolecules present over the bacterial membrane are responsible for this? Please explain and suggest me some literature.
Dear Manoranjan
the outer membrane of Gram-negative
bacteria acts as a permeability barrier for the cell. It is responsiple for preventing
molecules such as antibiotics, detergents and dyes from reaching the cytoplasmic
membrane.the outer membrane protects the cell by excluding nisin. Magnesium ions
serve to stabilize the lipopolysaccharide layer of the outer membrane. Chelating agents such as EDTA bind magnesium ions in the lipopolysaccharide layer and produce cells with increased susceptibility to antibiotics and detergents
Best regrades
Mohammed Al Marjani
Thank you very much Dr Marjani. Can you provide me some papers for reference?
I hope these helps
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC91573/
Understanding Pathogen Behaviour Virulence, Stress Response and Resistance
edited by Mansel Griffiths (page 468 in particular
Dear Manoranjan
this is some references
Kordel, M.; Schuller, F. and Sahl, H.G. (1989): Interaction of the pore forming
peptide antibiotics pep5, nisin and subtilin with non energized liposomes.
FEBS lett. 244, 99-102.
Hurst, A. (1983): Nisin and other inhibitory substances from lactic acid bacteria. In:
Antimicrobials in Food. P. 327-351. A.L. Branen and P.M. Davidsonn (ed.).
Series in food science. Vol. 10. Marcel Dekker, New York.
Hurst, A. and Hoover, D.G. (1983): Nisin. In: Amimicrobials in Foods. ed. Davidson,
P.M. and Branen, A.L. 2nd edn. Pp. 369-394. New York, Basel, HongKong:
Marcel Dekker.
Search for "Mechanisms of nisin resistance in Gram-positive bacteria " Hui Zhou,
Jun Fang,Yun Tian,Xiang Yang Lu
Mirna