Hi Guys,

The problem solved by adding some tween 20 as surfactant, also this problem was not observed using biological cells, thanks you in advance.

Have you tried to treat pdms with silane?

Try adding Pluronic F127 to modify the PDMS surface to be less sticky....see attached, includes recent review in nonbiofouling PDMS surface modification, which hopefully give other ideas.

...Positive type co-block polymers also include Tetronic, e.g Tetronic 1307. Addition of these type of Pluronic and/or Tetronic polymers to the sample matrix may also prevent the polystyrene particles sticking to each other; they are also relatively cheap :)

salam Yusef ,

thanks for your answer, I don't think silane can helps since it makes PDMS more hydrophobic , although hydrophobicity is desirable in SU-8 molding but it is the main reason of non specific protein adsorption.

فی امان الله

Hi John

thanks for comprehensive answer and useful links, Pluronic F127 is widely available in Iran and relativity cheap I'll check its feasibility and inform you about the results.

Thanks for your attention

Hi Guys,

The problem solved by adding some tween 20 as surfactant, also this problem was not observed using biological cells, thanks you in advance.