Why dose the hplc stadard sample have multiple peaks?

29 March 2024 0 5K Report

Experiments were conducted in the order 2-cyclohexen-1-one, cyclohexanone and mixture. The peak of cyclohexanone appears as one peak. Why does 2-cyclohexen-1-one have two large peaks and two small peaks? The mixture taken by mixing the two shows the same pattern, but the peak taken with cyclohexanone alone comes out normally. Is this a problem with the sample and not column washing?

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