I am trying to observe the oil body density of aleurone cells in mature rice seeds. I tried embedding it in Technovit 7100 resin using the protocol published by Ryo Matsushima (http://www.bio-protocol.org/e1239). I used a glass knife to section the resin blocks at 2µm thickness. However, the specimen turned into a powder-like consistency when sectioned.
Are there any possible solutions regarding this problem, maybe a modification in the protocol or cutting method?
Brent E Gowen
The resin has not infiltrated the sample, hence the powder-like consistency of the specimen. You can try extending all of the processing steps, especially once you start the Technovit infiltration. The cutting method is not the problem. Cheers. B
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