My blood agar plate turns to chocolate agar plate after being incubated at 37 degree Celsius for 24 hours. I cooled down the NA agar to 50 degree before adding the defibrinated sheep blood. What could I do?
I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...
02 March 2021 1,830 3 View
Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....
02 March 2021 7,890 2 View
An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...
02 March 2021 9,661 3 View
There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...
01 March 2021 831 2 View
L1 and L2 regularization
28 February 2021 4,187 3 View
Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...
26 February 2021 928 6 View
Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...
23 February 2021 9,956 3 View
We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...
23 February 2021 7,194 4 View
Lets consider a regression tree in which variance is 1.1065*e-10 and bias is 2.962e-13. Also the model RMSE on training set is 1.5e-5 and on training set is 1.2950e-5. Is the model is overfit or...
22 February 2021 7,755 2 View
Hi Colleagues- What do you think about the following methodological issues: 1. Is it methodologically wrong to formulate quantitative research questionnaire based on inductive reasoning? 2. Is it...
21 February 2021 4,634 6 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
01 March 2021 2,027 2 View
Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...
28 February 2021 7,292 3 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...
21 February 2021 7,260 4 View
I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...
16 February 2021 169 4 View
I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...
10 February 2021 5,927 1 View
I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...
09 February 2021 4,158 1 View
03 February 2021 1,111 5 View
I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...
01 February 2021 9,936 2 View
Please what are the composition of the solid agar could I use to grow the bacteria? what are the composition of the broth agar could I use to grow the bacteria and how to extract the natural...
26 January 2021 5,006 1 View