My blood agar plate turns to chocolate agar plate after being incubated at 37 degree Celsius for 24 hours. I cooled down the NA agar to 50 degree before adding the defibrinated sheep blood. What could I do?
After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...
08 August 2024 8,964 4 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
How Social Media Affects Your Mental Health
04 August 2024 6,961 3 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Do You Know Untreated TMJ Affects Your Brain And Entire Body?
04 August 2024 6,282 1 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
Please Help Us Urgently
01 August 2024 711 1 View
"How has Leader Sheikh Hasina's government allegedly responded to student protests, including the reported killing of over 500 students, as well as arrests, custody, remand, and the involvement of...
29 July 2024 2,181 7 View
My photocatalyst from solid waste red med. Dye is methylene blue My all parameter study is showing zero order. How to prove it further that the reaction in zero order?
29 July 2024 7,404 1 View
I know the rate law. But for my photo-catalytic experiments, first 90min is adsorption (dark) and next 150min is under UV light (total 240min). How to remove the adsorption part for calculation?...
21 July 2024 8,807 6 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...
12 July 2024 3,756 4 View
In the modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) protocols, can we replace blood agar with another culture medium?
10 July 2024 6,352 0 View
Hi I have a real problem growing Arabidopsis in hydroponic culture .I am using the bvoxes from Araponics and a Binder growth chamber with short day conditions and everything accurate .the seeds...
25 June 2024 9,151 6 View
I am trying to do gibson assembly cloning. I have two fragments for gibson assembly cloning (plasmid backbone and insert). After gibson assembly reaction I have transformed this in E. coli....
19 June 2024 6,404 4 View
Hi everyone. I need to do a lot of colony counting tomorrow and when I was doing it today I’ve noticed that most of the colonies are too transparent - I have to shine bright light on them all the...
12 June 2024 711 3 View
Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...
10 June 2024 4,152 1 View
I am working on antimicrobial effect of CBD (Cannabidiol). As it is insoluble in water, I prepared a stock of 500 mg/ml (in DMSO). Now I have two problems: 1- I can not dissolve more than 500 mg...
10 June 2024 9,564 6 View
Hi I am trying to revive Pseudomonas aeruginosa from frozen glycerol stock and also from cryobeads. But there was no bacterial growth. I used nutrient agar, tryptone soy agar to streak on. Also I...
09 June 2024 6,651 9 View