How does the N value be counted for gut content analysis?

I read some articles on mollusc and fish diet analysis. The formula for percentage frequency of occurrence stated above: %F0 = Nei/Ne x 100 and percentage numerical abundance : %NI : Ni/N x...

15 October 2020 4,568 3 View

Why is it necessary to vortex and incubate in dark the sample and DPPH solution and other DPPH questions?

Hi all, In the determination of Antioxidant capacity of fruit samples, I used DPPH assay and have a few questions regarding the DPPH assay 1) why is it necessary to vortex and incubate in dark...

30 August 2020 246 3 View

How to renew the Scopus index status of a journal?

Dear Researchers, Our journal (ISSN no. 2070-4658) was part of the Scopus index till 2017. Now, it is not in the Scopus index as the last two years no issue has been published. However, the...

24 August 2020 7,203 3 View

Hello,i extract my plants using rotary evaporator and i got crude extract.How should dilute it?i have no idea on how to dilute it?Can someone helpme?

dilute it in ethanol or dmso? which solution is better as i used 95% of ethanol as my extraction solvent?

23 March 2020 5,971 6 View

Can I set "20 uL reaction volume" in PCR cycler machine, while my actual sample is only 10 or 15 uL?

This is a stupid but quite technical question. We have a PCR machine that has 20 uL minimum reaction, so it can't be set into 15 or 10 uL in the machine. Is this going to affect the result...

22 March 2020 3,497 4 View

Is there any SOP to measure pharmaceutical clean room and if none can we develop our own method to measure the room size?

06 March 2020 866 1 View

How to test degree of polymerisation of aged kraft paper impregnated in oil?

kraft paper was impregnated in mineral oil at different temperatures and duration. my project is to get the DP value at different duration. in order to be measured by viscometer, the sample must...

20 January 2020 2,234 3 View

What is the equation to explain how one possible organic side product is formed in the synthesis of 1-bromobutane?

And show the chemical properties window of this side product. Explain how this product can be separated from the desired product. I have already identified that the possible organic side products...

29 December 2019 6,170 4 View

Validation for Intracerebroventricular Microinjection of Streptozotocin in Rats?

Hi, I'm planning to induce sporadic Alzheimer's disease (AD) in male Sprague Dawley rats (3 months old, 270-350g) through bilateral intracerebroventricular microinjection of 3 mg/kg streptozotocin...

25 November 2019 9,723 3 View

What is the meaning of negative activation energy?

I am prepared (Al, Co) co-doped TiO2 thin films by spray pyrolysis technique. The electrical measurement was performed by the four-probe point method, in the range of temperature 3ok (Room...

22 October 2019 526 4 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How can I find a Raman spectrum wavenumber corresponding to what compound?

I have several Raman spectra with very distinct peaks at certain wavenumbers, such as 277, 359, 466, 583, 623, 686, and 795cm-1. How can I identify them to what compound(s)? The RRUFF and others...

24 February 2021 363 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

Why the CHi^2 is close to zero with a bad fitting but the Rp and Rwp are still high?

I am refining my XRD data using in FullProf program. The intensity of the modulated X-ray peaks is almost flat. I tried to adjust the peak intensities but unsuccessful. What is the reason? I guess...

23 February 2021 2,304 4 View