Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View

Is it possible that the phosphorylated proteins mobilize faster than the non-phosphorylated ones?

The band downshifts on Western-blot, after kinase assay treatment.

23 December 2020 7,959 3 View

Species identification.Who can tell me the scientific names of these Gastropods?

Species identification. I've been doing research on communities of marine macrobenthos recently, but some of them can't be identified, which hinders my research. Who can help me identify these...

14 December 2020 8,357 3 View

Protocol for in vitro cytokine stimulation of immunized CD8+ T cells?

Hi, everyone: I want to determine whether IL-2 and IL-15 have different effects on immunized CD8+ T cell effector function. In this study, I immunize WT mice with peptide on day 0 and 7, and...

02 December 2020 9,413 3 View

Do I need to run a factor analysis on all the items used in the SEM to solve the multicollinearity issues?

Respected researchers, I conducted a SEM including 5 variables (two independent variables , two mediators, and a dependent variables), the correlations between these variables range vary from...

01 December 2020 1,338 5 View

How to extract RNA from small number (100-150 at most) of sorted cells?

I'm trying to test expression of a gene in sorted cells. I plan to extract RNA from sorted cells and to do RT-PCR (reverse transcription PCR). The problem is that from sorting, I can only collect...

21 November 2020 5,467 2 View

After place the crudes on an column spectroscopy (sorry, column chromotograph), is it approprate to touch or move the crude sample anymore?

As the title has asked. For instence, using metal stick or long glass tube to get rid of the bubbles in columns.

05 November 2020 5,946 4 View

Transformation of Bacillus subtilis?

Colonies appeared after transformation of pHT01 plasmid into Bacillus subtilis, and band was confirmed by performing colony PCR. However, if the colony is picked and grown (in LB+antibiotics) and...

27 October 2020 609 4 View

B cell isolation from mice spleen using Meltinyi negative kit?

Hi, guys, Do you have problem using B cell negative selection kit form Meltinyi? Sometime the B cell is pure, CD19+>95%, but sometimes it lower than 90%.

19 October 2020 5,160 3 View

How to separate exosome and subcellular organelle (eg. ER) of mouse liver?

I want to do tissue subcellular fractionation of mouse liver. According to the method, the speed to separate ER is over 200,000g for 120 min (Song et al., 2006). However, liver exosome isolation...

12 October 2020 4,929 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View