How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Where can I find atmospheric measurement profiles (temperature, density, etc.) between 300-800 km altitude?

I am investigating thermospheric mass density profiles between 300-800 km altitude. I would like to compare the variability with other parameters, including temperature, electron content,...

01 March 2021 5,902 3 View

Possible problem for showing 0.2 sun intensity instead of 1 sun intensity in AAA class Newport solar simulator?

We have a AAA class Newport solar simulator. We changed the Xenon Arc Lamp recently (XBO450W, Make:OSRAM) as the lifetime got over. However, the intensity is coming to be only 0.2 sun instead of 1...

14 February 2021 9,998 3 View

Which sphygmomanometer is suitable for teaching practicals in Physiology, Android or Non-Mecurial LED Sphygmomanometer??

Recently MCI now NMC has made it mandatory to use only Non mercury Sphygmomanometer for teaching practicals to MBBS students. So I want ask which is technically better option, Android or...

03 February 2021 3,795 4 View

Is it necessary to include orbital dynamics (translational) in attitude dynamics (rotational) problem?

I want to simulate satellite attitude control with a quaternion attitude representation. thrusters and reaction wheels are the main AOCS controllers. is it Ok to omit orbit dynamics (orbit...

31 January 2021 5,094 2 View

Why and how to avoid negative values in optical density by spectrophotometry when measurements corrected by blank solvent?

I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...

20 January 2021 1,542 4 View

In the Venusian atmosphere why doesn't Nitrogen which is much lighter Carbon Dioxide float to the upper regions?

Carbon dioxide on Earth weighs 44 g/mol and has a density of around 1.98 kg/m3, about 1.53 times that of air. Nitrogen gas weighs 28 g/mol and has a density of 1.2506 g/L. During limnic eruptions...

15 January 2021 8,733 4 View

Can cosine of solar zenith angle be negative?

I am trying to find the cosine of sun elevation angle on slope terrain, I found the values between -0.9 to 0.9. Should the value be set to 0 - 0.9 or the negative value acceptable?

24 December 2020 4,027 4 View

How much salt in a m^3 of water would be needed to prevent evaporation ?

Assume you have 1 cubic meter of water with 1 square meter of surface exposed to the sun. If you kept adding salt to the solution, is there a point at which evaporation would cease? Assuming the...

22 November 2020 5,444 1 View

Possibility of using a reference frame to account for dark matter, or summing the kinetic energies in a galaxy?

Dark matter is some extra energy that we observe which bends spacetime such that a star orbiting a galaxy will appear to have escape velocity but will remain in a stable orbit. When solving for...

20 November 2020 1,350 4 View

How adsorbed Mercury (II) react with HCl and HNO3 in water?

Case: Hg2+ is adsorbed on a titanium carbide and 0.1 M HCl/HNO3 is used for the desorption of Hg2+. During the reaction, the solution turns into a pale yellow color. I want to understand what...

16 November 2020 5,770 3 View