Recently, I have been screened several types of immortalised cell lines (neuronal, N27 and HT-22, and cancer, HT1080 and U251), but TrkB expression was not found in any of them. The antibody was good that detected TrkB expressions in mouse brain tissue and primary cortical neurons.
this is interesting, and, assuming that the antibody was tested side-by-side and in the same conditions (4% PFA fixative, same time) in ICC, looks real. Immortalized cells undergo multiple epigenetic changes, this is discussed and published, read about epigenetic regulation of TrkB promoter. One likely reason is epigenetic silencing of promoter. Then, immortalized cels are... immortal, therefore there is no pressure on cells to survive as immortalization took care of enabling proliferation and survival. TrkB is important for survival as well as maturation, and in the absence of pressure its presence likely becomes redundant. E.g., Article TrkB/BDNF Signaling Regulates Photoreceptor Progenitor Cell ...
It is helpful to know and highlights the "value" of immortalized lines for neuroscience studies.- Badges
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