The absorbance is very high. Most devices I know measure only reliable up to 1. Is it possible that the peaks are artefacts? What are the limitations of your photometer. Furthermore particles are known to scatter in the UV. Than it could be scattering and not absorption.
Sir, what are you referencing to artefacts here? I did not understand.
It could have been scattering cause I do suspect presence of certain carbonaceous material apart from my carbon dots.
Dear Ronak,
I agree fully with the answers of the answerers (see above). BUT
- your question is too vague for answerers. Reformulate your question and give more infos about Cdots and particle size and solvent and typ of UV-Vis spectrometer. Dilute in order to have a absorbance up to 1.
- Typ of cuvettes (quartz ?) and give the name of your solvent see the cut-off of it.
Please read the two
https://scholar.google.de/scholar?q=carbon+dots+spectroscopy&hl=de&as_sdt=0&as_vis=1&oi=scholart&sa=X&ei=h0pjVanZDcS6sQHTtoGgDw&ved=0CB4QgQMwAA
http://cdn.intechopen.com/pdfs-wm/37655.pdf
Good luck and thank you in advance.
JRG
Thank You Mr. Grezes
The Cdots are a part of a composite with mesoporous silica The solvent is PBS (phosphate buffer saline) The UV/VIs spec was a single beam type. Cuvettes were quartz. I havent diluted the solution as the silica part of the composite is too low in concentration.
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