Why do I have high and decreasing RFU over the first 10 QPCR Cycles?

11 March 2021 1 5K Report

I have been consistently getting very high RFU values at the beginning of most of my qPCR runs. The RFU then drops off over the first 10-15 cycles and then increases as the thing I am amplifying amplifies (See attached image). I'm using a BioRad CFX 96 with PowerUp Sybr Green Super Mix by Thermo. Any idea what is going on here?

Antethar Jabar

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