I have initial grape and blackcurrant powders, that have gone through polyphenol extraction process with 30% ethanol and 0.1% acetic acid. I have conducted a Folin assay on them to get the polyphenol content. At the next stage, I encapsulated the extracts with gum Arabic and spray-dried them. The spray-dried powders have gone through the same extraction condition with the same percentage of ethanol and acetic acid. The supernatants were collected for a Folin assay to get the polyphenol content. To calculate the encapsulation efficiency, I have divided the values of TPC in the powders by the TPC in the initial extracts and multiplied by 100. Strangely, I have gained results of more than 100, such as 200%, 300%, etc. Moreover, I have prepared dilutions of my samples to get absorbance within the standard curves. Could you please guide me on what is wrong with my measurements? Or if you could kindly share some hints in this regard. Thank you.