Why dind't TGFb1 show western blot?

26 November 2014 1 7K Report

I recently have trouble with detecting TGFb1 expression in fibroblasts cell lysates using western blot... It just didn't show. My actin bands showed well....Has anybody done that before?

Amira Fakih

maybe you should optimize the primary and secondary antibody dilutions

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Science method

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