Protocol used: Article RNA Isolation from Embryonic Zebrafish and cDNA Synthesis fo...
After isolation: Qubit kit was used for quantification (HS): below the detectable limit
No gel-like pellet on the bottom of the tube was observed during the isolation procedure.
please give some suggetions
RNA can be challenging to isolate. Sounds like you have a very detailed protocol to follow, did you do anything differently than specified?
Common issues are: not IMMEDIATELY flash-freezing tissue samples, allowing samples to thaw before adding Beta-mercaptoethanol, not thoroughly disrupting the tissue, etc.
Good luck!
Dear Dr. Burnette,
performed the work according to the prescribed protocol, but, we used rohu eggs in this case.
Beta-mercaptoethanol has not been used.
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