Production of phenolic compounds in the medium is a disturbance or contamination during plant tissue culture, but how are these compounds produced? Is it due to any nutrient scarcity in the medium?
The crucial fact to appreciate in this context is that tissue culture is an extraordinarily stressful treatment of the plant tissue, regardless of whether full nutrition is provided or not. Excision, osmoticum, hormones etc. are treatments used during tissue culture that can cause severe stress. It is well known that wounding, pathogen, drought, salt, cold and a number of other stresses induce the pathway(s) that produce phenolic compounds in plants. I believe that the specific combination of stress factors in the tissue culture conditions (culture medium, light, temperature etc.) and the species/ explants being cultured, result in production phenolics while others do not.
I am not aware of the full literature on it. However, you should be able to piece together at least a partial picture of it if you do a lit.
best of luck
The crucial fact to appreciate in this context is that tissue culture is an extraordinarily stressful treatment of the plant tissue, regardless of whether full nutrition is provided or not. Excision, osmoticum, hormones etc. are treatments used during tissue culture that can cause severe stress. It is well known that wounding, pathogen, drought, salt, cold and a number of other stresses induce the pathway(s) that produce phenolic compounds in plants. I believe that the specific combination of stress factors in the tissue culture conditions (culture medium, light, temperature etc.) and the species/ explants being cultured, result in production phenolics while others do not.
I am not aware of the full literature on it. However, you should be able to piece together at least a partial picture of it if you do a lit.
best of luck
Dear Anju
The first response to the stress in plant is production of phenol, you can try PVP to inhibe phenolic compunds
Good luck
It is well known that phenolic compounds are the building units of lignin. So, it is produced in case of wounding followed by peroxidases that causes polymerization of these phenolics to form lignin. The plant tends to form lignin to protect the wound from the surround environment. The tissue culture conditions are not as the normal conditions, where the plant cells protected well from the surround environemnt. So, it tend to synthesis phenolics thus lignin in the tissue culture media.
I agree that production of phenolic compounds is a response of the plant tissue to streeses, such as wounds and in vitro culture. I also observed that different sugarcane genotypes react to the stresses differently, while some produce large amounts of phenolic compounds, some produced a little. In addition, more frequent transfer of explant tissue to fesh media may also help reduce the production of phenolic compounds.
Silver nitrate is a good choice to get rid of the phenolic compounds produced during in vitro culture.......frequent sub culturing can also help.....
Dear, it is not silver chloride...it is silver nitrate.....the concentration is needed to be optimised as per needs.....it is usually between 2-5 mg/l.......
Phenolic compunds are produced as a response to combination of stresses that the plant obtain mostly in in vitro conditions and silver nitrate or activated charcoal may be used at desired quantity as a preventive against phenolic compounds
Add charcoal to the medium. Get rid of phenolics problem.. Phenolics are the result of secondary metabolism in plant tissue. As it is in glass tube, it will be in stress. Stress trigger secondary metabolism. This may be the cause..
Phenolics have been serious problem in guava tissue culture and PVP have been used to control effectively.
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