Will they show more resistance if I test them further?
Colonies within the zone of inhibition
Good day.
I'm thinking you're referring to inhibition test of a drug, correct? Or is about "satellite" colonies in a transformation?
Whichever the case is, cells growing within this kind of zone are not more susceptible, they're equally susceptible, as the drug in the media within that area of inhibition is more likely degraded.
Furthermore, if you get cells growing in media that they're not supposed to grow, what you're may be getting are mutant cells, that got "in someway" the capabilities to resist and overcome the selection pressure you were imposing with the drug, and these cells would be actually more resistant, not more susceptible.
Hope that helps in some way. Keep us informed!
Cheers
hello
Pueden ser mutantes resistentes, célular que genéticamente han conferido resistencia al antibiótico. descarta una contaminación o un inóculo muy grande, debe ser 0.5 escala Mac Farland.
Hello..I agree with Cañas-Villamar, they are satellite colony. These are dormant microbes, when antibiotic degraded by actually resistant microbes or due to abiotic condition for prolong incubation they grow. I understood you are doing some transformation experiments, with that respect this happens mostly in ampicillin antibiotic case try to use Kanamycin which may get rid of this problem at some extent.
Colonies that grow within a zone of inhibition are NOT susceptible to the antibiotic used at that particular concentration. This is one way in which you can select for resistant mutants to the specific antibiotic. It is also possible that your plate is contaminated by a non-susceptible strain.
I meant resistant no susceptible, sorry, Thank you all for your help. Really appreciate it.
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