I have been using SYGreen dye for all of my qPCR run and have never seen a sagging after plateaue phase. However, lately, while using a new primer I am getting a drooping plateaue phase. Initially I thought it to be a "hook effect" as I am working with a SINE (transposon). Therefore, I have diluted my samples 10 folds and decresed the cycle numbers but nothing helped. If anyone faced the same challenge kindly help me solving the problem.