In an in-vivo study against MCF-7 Breast cancer, it was observed that the response to the targeted drug containing surface engineered silver nanoparticles was highly different in three mice as compared to other three of a group of 6 NOD-SCID mouse. Why?
If your mice groups are different if you mean that, the impaired T and B cell lymphocyte is the difference to give varied results.
Dear researcher,
Various issues could be affected the results, such as the stage of tumor, inappropriate drug injection (accurate tumore injection or IV injection without technical errors), the size of tumor (the same size and weight should be considered as am accurate comparison), diet conditions of mice should be control for all, and the prepared drug concentration check must be exactly done before administration.
The erratic behavior of the silver particles indicates to me they have Non specific binding issues. you will need to re examine your ratio of ceils to particles to reduce NSB
Thanks for the answer. However, these silver nanoparticles were specifically functionalized for hormone targeted delivery at the cancer site
It was better if you would have put all info and details relevant to the query the first time and not in piece-meal! Drs. Ashgar and Henry's responses still hold good and guide you!
Thank you sir but at times, I feel, one finds it difficult to put all the details of the study. Also, I needed to know all possible reasons and get checkpoints if not explored. Sorry for any discomfort. Actually, the testing has been done at ACTREC, Tata memorial Mumbai with standard protocols. The drug has been formulated at my end. Still expecting possible causes for this indifferent response
Specifically functionalized Silver particles and non specific binding are unrelated. What NSB means, the particles have encountered more NSB cells than the target. Adjust your particle/cell ratio
Thank you sir
Please guide how it can be done
Following is the data provided by the lab corresponding to my Formulation of silver nanoparticles functionalized for targeting the hormones expressed at the MCF-7 Breast Cancer site in NOD-SCID mice. This in-vivo study was conducted using the Human tumor Xenograft Model.
Tumor Volume Group C
Days Mouse 1 Mouse 2 Mouse 3 Mouse 4 Mouse 5 Mouse 6
1 0.07 0.07 0.07 0.07 0.08 0.08
5 0.12 0.13 0.10 0.07 0.19 0.07
9 0.35 0.54 0.06 0.06 0.85 0.09
12 0.60 0.76 0.15 0.05 1.60 0.17
15 1.30 1.34 0.28 0.10 2.52 0.31
18 2.46 3.36 0.66 0.19 5.28 0.96
21 3.61 4.17 0.75 0.21 6.91 0.99
Sir, also I am not able to understand the reason behind varied NSB among the same type of mice with the same type of formulation
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